Name β-D-Glucose:oxygen 1-oxydoreductase
Appearance Yellowish amorphous powder lyophilized
Activity: Grade I, 180 U/mg-solid or more
Contaminants: Catalase ≤ 5 x 10-3 %
Stabilizers: Potassium gluconate, sodium glutamate
Stability: Stable at −20°C for at least 12 months
Molecular weight: approx. 153,000
Michaelis constants: 3.3×10-2 M (β-D-Glucose) 6.1×10-2M (2-Deoxyglucose)
Structure: Glycoprotein with 2 moles of FAD
Inhibitors: p-Chloromercuribenzoate, heavy metal ions
Optimum pH: 4.5
Optimum temperature: 40−50°C
pH Stability: pH 4.5−6.0 (30°C, 20hr)
Thermal stability: below 50°C (pH 5.7, 1hr)
EC # 1.1.3.4